1
عمومی::
واکنش زنجیرهای پلیمراز، واکنش زنجیره ای پلیمراز، واکنش زنجیره ای پلیمراز، واکنش زنجیره ای پلیمراز، واکنش زنجیرهای پلیمراز
Commercial kits are available that use polymerase chain reaction (PCR) amplification (see next paragraph) with an enzyme‐linked immunosorbent assay (ELISA); they have been used to detect mecA in blood cultures containing S. aureus or different carbapenemase resistance genes in cultures of the Enterobacteriaceae.
PCR methods for the amplification of DNA sequences have been used to detect specific antimicrobial resistance genes or identify pathogens in clinical samples.
In the standard PCR method, samples containing DNA are added to a reagent tube containing forward and reverse primers specific for the gene to be amplified, a deoxyribonucleotide triphosphate mix, and Taq polymerase.
If the genetic basis of resistance is known, PCR can be used to detect the genes whose expression may decrease the susceptibility of bacteria to particular antibiotics, such as the mecA gene for methicillin resistance in MRSA.
PCR methods are only useful for detecting resistance genes whose sequence is known.،Tests should include CSF cell count, protein, glucose, Gram stain, and culture, but any previous antibiotic dosing can diminish sensitivity, although polymerase chain reaction (PCR) testing using amplification may be able to identify small amounts of DNA in a sample.
Viral PCR, immunoglobulin, fungal antigens, and cultures can also be considered.
be drawn for viral DNA PCR as well as the routine biochem- istry, culture, and cell count.
Paired samples are recommended to allow later comparison if CSF PCR is unhelpful.
Brain biopsy was considered the gold standard but is now a last option, after CSF PCR, culture, and serology have proven negative.259،Viral culture can be obtained from an unroofed vesicle, or polymerase chain reaction or direct fluorescent antibody testing can be performed, on swabbed tissue.
PCR testing of spinal fluid is the testing modality of choice for HSV when suspected, and the viral DNA can be detected within the first 24 hours, with results remaining positive for a week or longer.
testing, or PCR testing of vesicle fluid may be appropriate in patients with atypical illness or severe disease.
Consider advanced testing when CNS infection is suspected, which may include an MRI of the brain, lumbar puncture, and/or PCR testing.
Confirm these rare infections using acute serologic testing to identify antibodies or reverse transcriptase polymerase chain reaction of the virus.،Tests should include CSF cell count, protein, glucose, Gram stain, and culture, but any previous antibiotic dosing can diminish sensitivity, although polymerase chain reaction (PCR) testing using amplification may be able to identify small amounts of DNA in a sample.
Viral PCR, immunoglobulin, fungal antigens, and cultures can also be considered.
be drawn for viral DNA PCR as well as the routine biochem- istry, culture, and cell count.
Paired samples are recommended to allow later comparison if CSF PCR is unhelpful.
Brain biopsy was considered the gold standard but is now a last option, after CSF PCR, culture, and serology have proven negative.259،Commercial kits are available that use polymerase chain reaction (PCR) amplification (see next paragraph) with an enzyme‐linked immunosorbent assay (ELISA); they have been used to detect mecA in blood cultures containing S. aureus or different carbapenemase resistance genes in cultures of the Enterobacteriaceae.
PCR methods for the amplification of DNA sequences have been used to detect specific antimicrobial resistance genes or identify pathogens in clinical samples.
In the standard PCR method, samples containing DNA are added to a reagent tube containing forward and reverse primers specific for the gene to be amplified, a deoxyribonucleotide triphosphate mix, and Taq polymerase.
If the genetic basis of resistance is known, PCR can be used to detect the genes whose expression may decrease the susceptibility of bacteria to particular antibiotics, such as the mecA gene for methicillin resistance in MRSA.
PCR methods are only useful for detecting resistance genes whose sequence is known.
واژگان شبکه مترجمین ایران
2
مکانیک::
واکنش زنجیره ای پلیمراز
trophoresis), dielectrophoresis, polymerase chain reaction (PCR) and gene delivery and further various sensing and detection techniques like electrochemical sensing, optical sensing, mass based sensing and surface plasmon resonance (SPR) sensing etc.
* all required molecular assaysteps,from cellular lysis to�DNA polymerase chain reaction amplification, amplicons digestion, and microarray hybridization on a plastic support The low-temperature, pressure-free assembly and bonding of sTPE material on the flat polydo-olefin thinsubstrateoffer a pertinent solution for simpleand efficient loadingand storage of the required on-CDboard elements.This wasdemonstratedthroughthe Integration and the conditioning of microbeads, magnetic discs, and dried enzyme species.
T H/m is the permeability of vacuum, and pcr is the relative
The fast temperature change allows a short thermal cycling period in reactions such as polymerase chain reaction ( PCR ), Furthermore, the small size allows a more homogeneous temperature distribution in the reactor, and thus increases the efficiency of the reaction.
Native silicon and silicon nitride, for instance inhibit PCR in the microscale.
واژگان شبکه مترجمین ایران